goat polyclonal gapdh Search Results


goat polyclonal anti smad1 5  (OriGene)
90
OriGene goat polyclonal anti smad1 5
Goat Polyclonal Anti Smad1 5, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gapdh  (OriGene)
89
OriGene anti gapdh
Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels <t>of</t> <t>PD‐L1</t> (top) and <t>GAPDH</t> (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.
Anti Gapdh, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gapdh/product/OriGene
Average 89 stars, based on 1 article reviews
anti gapdh - by Bioz Stars, 2026-03
89/100 stars
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anti-gapdh goat polyclonal  (Linaris Biologische Produkte)
90
Linaris Biologische Produkte anti-gapdh goat polyclonal
Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels <t>of</t> <t>PD‐L1</t> (top) and <t>GAPDH</t> (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.
Anti Gapdh Goat Polyclonal, supplied by Linaris Biologische Produkte, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-gapdh goat polyclonal/product/Linaris Biologische Produkte
Average 90 stars, based on 1 article reviews
anti-gapdh goat polyclonal - by Bioz Stars, 2026-03
90/100 stars
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goat polyclonal anti-gapdh  (Clinisciences)
90
Clinisciences goat polyclonal anti-gapdh
Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels <t>of</t> <t>PD‐L1</t> (top) and <t>GAPDH</t> (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.
Goat Polyclonal Anti Gapdh, supplied by Clinisciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal anti-gapdh/product/Clinisciences
Average 90 stars, based on 1 article reviews
goat polyclonal anti-gapdh - by Bioz Stars, 2026-03
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polyclonal goat anti gapdh  (OriGene)
90
OriGene polyclonal goat anti gapdh
Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels <t>of</t> <t>PD‐L1</t> (top) and <t>GAPDH</t> (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.
Polyclonal Goat Anti Gapdh, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal goat anti gapdh/product/OriGene
Average 90 stars, based on 1 article reviews
polyclonal goat anti gapdh - by Bioz Stars, 2026-03
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gapdh goat polyclonal antibody  (OriGene)
89
OriGene gapdh goat polyclonal antibody
Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels <t>of</t> <t>PD‐L1</t> (top) and <t>GAPDH</t> (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.
Gapdh Goat Polyclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh goat polyclonal antibody/product/OriGene
Average 89 stars, based on 1 article reviews
gapdh goat polyclonal antibody - by Bioz Stars, 2026-03
89/100 stars
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gapdhs goat polyclonal antibody af6276  (Cedarlane)
90
Cedarlane gapdhs goat polyclonal antibody af6276
Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels <t>of</t> <t>PD‐L1</t> (top) and <t>GAPDH</t> (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.
Gapdhs Goat Polyclonal Antibody Af6276, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdhs goat polyclonal antibody af6276/product/Cedarlane
Average 90 stars, based on 1 article reviews
gapdhs goat polyclonal antibody af6276 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels of PD‐L1 (top) and GAPDH (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.

Journal: EMBO Reports

Article Title: MLLT 6 maintains PD ‐L1 expression and mediates tumor immune resistance

doi: 10.15252/embr.202050155

Figure Lengend Snippet: Genotyping nine RKO MLLT6 knockout (KO) clones. Sequencing reads from individual clones aligned to reference sequence of MLLT6 (top row), with sgRNA target site and PAM motif (blue) and insertion‐deletion (indel) mutations as indicated (red). MLLT6 transcript levels (± SD) in RKO mock and MLLT6 KO clones normalized to expression in control cells from four technical replicates. Immunoblot showing total cellular protein levels of PD‐L1 (top) and GAPDH (bottom) in RKO MLLT6 KO clones and mock control cells. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 and MLLT6 transcript levels (± SD) in RKO mock, MLLT6 KO, and MLLT6 KO + MLLT6 BAC normalized to control cells (Student's t ‐test; * P < 0.05, ** P < 0.01; n = 3; biological replicates; df = 2). Source data are available online for this figure.

Article Snippet: Western Blot: rabbit anti‐PD‐L1 (E1L3N) XP (Cell Signaling Technology, 13684), rabbit anti‐PD‐L1 (Thermo Fisher scientific, PA5‐28115), goat anti‐GAPDH (Acris Antibodies, AP16240PU‐N), rabbit anti‐IFNGR1 (Cell Signaling Technology, 34808), rabbit anti‐IFNGR2, C‐term (Gene Tex, GTX81601), rabbit anti‐JAK1(6G4) (Cell Signaling Technology, 3344), rabbit anti‐JAK2 (D2E12) (Cell Signaling Technology, 3230), rabbit anti‐IDO1 (Gene Tex, GTX113753), rabbit anti‐HLA‐DRB1 (N1C3) (Gene Tex, GTX104919), rabbit anti‐HLA‐DRA (N2C3) (Gene Tex, GTX113732), rabbit anti‐CD74 (Gene Tex, GTX110477), rabbit anti‐GBP5 (Gene Tex, GTX106994), rabbit anti‐STAT1 (Cell Signaling Technology, 9172), rabbit anti‐phospho‐STAT1 (Tyr701) (D4A7) (Cell Signaling Technology, 7649), IRDye 680LT donkey anti‐goat IgG (LI‐COR Biosciences, 926‐68024), IRDye 800 CW donkey anti‐rabbit IgG (LI‐COR Biosciences, 926‐32213).

Techniques: Knock-Out, Clone Assay, Sequencing, Expressing, Western Blot

PD‐L1 cell surface presentation (percentage ± SD) in RKO mock control (black) or monoclonal MLLT6 knockout cells (gray) (Student's t ‐test; ** P < 0.01; n = 3 biological replicates; degrees of freedom (df) = 2). MLLT6 BAC mediated rescue in U2OS cells (left) showing percentage ± SD of PD‐L1 cell surface presentation in mock control (black), MLLT6 polyclonal knockout (light gray) and MLLT6 knockout + BAC cells (dark gray) (Student's t ‐test; * P < 0.05; n = 3 biological replicates; df = 2). Representative flow cytometry plots (right) of PD‐L1 cell surface presentation after staining with anti‐PD-L1 antibody (top right, bottom left, bottom right) and isotype control (top left). Percentages of cells in the PD‐L1 + gate as indicated. Total cellular protein levels of PD‐L1 (top) and GAPDH (bottom) in monoclonal RKO MLLT6 knockout, mock control or MLLT6 knockout + BAC cells visualized by immunoblotting. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 transcript levels in U2OS mock (black) and polyclonal MLLT6 knockout lines (gray) treated with and without IFN‐γ normalized to expression in mock U2OS cells without IFN‐γ treatment (ANOVA; ** P < 0.01; n = 4 biological replicates; df = 12). Representative flow cytometry plots of PD‐L1 cell surface presentation in the presence and absence of IFN‐γ in mock, polyclonal MLLT6 knockout and PD‐L1 knockout cells. Percentages of cells in the PD‐L1 + gate after staining with anti‐PD-L1 antibody or isotype control as indicated. Total cellular protein levels of PD‐L1 (top) and GAPDH (bottom) in U2OS polyclonal MLLT6 knockout or mock control cells with and without IFN‐γ stimulation. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. Source data are available online for this figure.

Journal: EMBO Reports

Article Title: MLLT 6 maintains PD ‐L1 expression and mediates tumor immune resistance

doi: 10.15252/embr.202050155

Figure Lengend Snippet: PD‐L1 cell surface presentation (percentage ± SD) in RKO mock control (black) or monoclonal MLLT6 knockout cells (gray) (Student's t ‐test; ** P < 0.01; n = 3 biological replicates; degrees of freedom (df) = 2). MLLT6 BAC mediated rescue in U2OS cells (left) showing percentage ± SD of PD‐L1 cell surface presentation in mock control (black), MLLT6 polyclonal knockout (light gray) and MLLT6 knockout + BAC cells (dark gray) (Student's t ‐test; * P < 0.05; n = 3 biological replicates; df = 2). Representative flow cytometry plots (right) of PD‐L1 cell surface presentation after staining with anti‐PD-L1 antibody (top right, bottom left, bottom right) and isotype control (top left). Percentages of cells in the PD‐L1 + gate as indicated. Total cellular protein levels of PD‐L1 (top) and GAPDH (bottom) in monoclonal RKO MLLT6 knockout, mock control or MLLT6 knockout + BAC cells visualized by immunoblotting. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. PD‐L1 transcript levels in U2OS mock (black) and polyclonal MLLT6 knockout lines (gray) treated with and without IFN‐γ normalized to expression in mock U2OS cells without IFN‐γ treatment (ANOVA; ** P < 0.01; n = 4 biological replicates; df = 12). Representative flow cytometry plots of PD‐L1 cell surface presentation in the presence and absence of IFN‐γ in mock, polyclonal MLLT6 knockout and PD‐L1 knockout cells. Percentages of cells in the PD‐L1 + gate after staining with anti‐PD-L1 antibody or isotype control as indicated. Total cellular protein levels of PD‐L1 (top) and GAPDH (bottom) in U2OS polyclonal MLLT6 knockout or mock control cells with and without IFN‐γ stimulation. Numbers indicate relative band intensities of PD‐L1 protein normalized to GAPDH. Source data are available online for this figure.

Article Snippet: Western Blot: rabbit anti‐PD‐L1 (E1L3N) XP (Cell Signaling Technology, 13684), rabbit anti‐PD‐L1 (Thermo Fisher scientific, PA5‐28115), goat anti‐GAPDH (Acris Antibodies, AP16240PU‐N), rabbit anti‐IFNGR1 (Cell Signaling Technology, 34808), rabbit anti‐IFNGR2, C‐term (Gene Tex, GTX81601), rabbit anti‐JAK1(6G4) (Cell Signaling Technology, 3344), rabbit anti‐JAK2 (D2E12) (Cell Signaling Technology, 3230), rabbit anti‐IDO1 (Gene Tex, GTX113753), rabbit anti‐HLA‐DRB1 (N1C3) (Gene Tex, GTX104919), rabbit anti‐HLA‐DRA (N2C3) (Gene Tex, GTX113732), rabbit anti‐CD74 (Gene Tex, GTX110477), rabbit anti‐GBP5 (Gene Tex, GTX106994), rabbit anti‐STAT1 (Cell Signaling Technology, 9172), rabbit anti‐phospho‐STAT1 (Tyr701) (D4A7) (Cell Signaling Technology, 7649), IRDye 680LT donkey anti‐goat IgG (LI‐COR Biosciences, 926‐68024), IRDye 800 CW donkey anti‐rabbit IgG (LI‐COR Biosciences, 926‐32213).

Techniques: Knock-Out, Flow Cytometry, Staining, Western Blot, Expressing

Scatter plots of pairwise comparisons of transcript expression in U2OS cells. Plots describe log 2 fold change in transcript expression in mock and polyclonal MLLT6 knockout cells treated with and without IFN‐γ as indicated. Horizontal axis shows log 2 fold change in transcript expression and vertical axis represents statistical significance (log 10 of P ‐value, n = 3) with candidates nominated for further validation (red). Correlation plots describing log 2 fold change of transcripts in RNA sequencing ( x ‐axis) and NanoString ( y ‐axis) measurements in mock cells ± IFN‐γ (left) and MLLT6 knockout cells with IFN‐γ (right). Transcripts previously described to have a role in tumor immunity (red) and PD‐L1 (blue) are represented on the plots as indicated. Changes (log 2 fold) in transcript expression (left) of select immune‐related genes in U2OS mock or MLLT6 knockout cells treated with or without IFN‐γ and total protein levels measured by immunoblotting (right). Numbers indicate band intensities normalized to GAPDH. Source data are available online for this figure.

Journal: EMBO Reports

Article Title: MLLT 6 maintains PD ‐L1 expression and mediates tumor immune resistance

doi: 10.15252/embr.202050155

Figure Lengend Snippet: Scatter plots of pairwise comparisons of transcript expression in U2OS cells. Plots describe log 2 fold change in transcript expression in mock and polyclonal MLLT6 knockout cells treated with and without IFN‐γ as indicated. Horizontal axis shows log 2 fold change in transcript expression and vertical axis represents statistical significance (log 10 of P ‐value, n = 3) with candidates nominated for further validation (red). Correlation plots describing log 2 fold change of transcripts in RNA sequencing ( x ‐axis) and NanoString ( y ‐axis) measurements in mock cells ± IFN‐γ (left) and MLLT6 knockout cells with IFN‐γ (right). Transcripts previously described to have a role in tumor immunity (red) and PD‐L1 (blue) are represented on the plots as indicated. Changes (log 2 fold) in transcript expression (left) of select immune‐related genes in U2OS mock or MLLT6 knockout cells treated with or without IFN‐γ and total protein levels measured by immunoblotting (right). Numbers indicate band intensities normalized to GAPDH. Source data are available online for this figure.

Article Snippet: Western Blot: rabbit anti‐PD‐L1 (E1L3N) XP (Cell Signaling Technology, 13684), rabbit anti‐PD‐L1 (Thermo Fisher scientific, PA5‐28115), goat anti‐GAPDH (Acris Antibodies, AP16240PU‐N), rabbit anti‐IFNGR1 (Cell Signaling Technology, 34808), rabbit anti‐IFNGR2, C‐term (Gene Tex, GTX81601), rabbit anti‐JAK1(6G4) (Cell Signaling Technology, 3344), rabbit anti‐JAK2 (D2E12) (Cell Signaling Technology, 3230), rabbit anti‐IDO1 (Gene Tex, GTX113753), rabbit anti‐HLA‐DRB1 (N1C3) (Gene Tex, GTX104919), rabbit anti‐HLA‐DRA (N2C3) (Gene Tex, GTX113732), rabbit anti‐CD74 (Gene Tex, GTX110477), rabbit anti‐GBP5 (Gene Tex, GTX106994), rabbit anti‐STAT1 (Cell Signaling Technology, 9172), rabbit anti‐phospho‐STAT1 (Tyr701) (D4A7) (Cell Signaling Technology, 7649), IRDye 680LT donkey anti‐goat IgG (LI‐COR Biosciences, 926‐68024), IRDye 800 CW donkey anti‐rabbit IgG (LI‐COR Biosciences, 926‐32213).

Techniques: Expressing, Knock-Out, RNA Sequencing Assay, Western Blot